Elucidating the Stoichiometries of Host-Pathogen Protein Interactions with Mass Photometry.
Methods in molecular biology (Clifton, N.J.)
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| Abstract | Mass photometry (MP) is a single molecule technique that enables the characterization of individual proteins. Here we show a detailed workflow using the Refeyn One to investigate molecular complexes, using the M53 protein, a plasminogen-binding group A streptococcal M-like protein (PAM), and human plasminogen as exemplar proteins. The methodology described herein confirmed a 1:1 binding stoichiometry for the M53-plasminogen complex. Additionally, MP was used to identify the oligomerization state, homogeneity, purity, and approximate molecular weights of each protein. |
| Year of Publication | 2023
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| Journal | Methods in molecular biology (Clifton, N.J.)
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| Volume | 2674
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| Pages | 201-208
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| Date Published | 12/2023
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| ISSN | 1940-6029
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| DOI | 10.1007/978-1-0716-3243-7_13
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| PubMed ID | 37258969
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