Next-generation transgenic mice for optogenetic analysis of neural circuits.

Front Neural Circuits

Authors	Brent Asrican George Augustine Ken Berglund Susu Chen Nick Chow Karl Deisseroth Guoping Feng Bernd Gloss Riichiro Hira Carolin Hoffmann Haruo Kasai Malvika Katarya Jinsook Kim John Kudolo Li Lee Shun Lo James Mancuso Masanori Matsuzaki Ryuichi Nakajima Li Qiu Gregory Tan Yanxia Tang Jonathan Ting Sachiko Tsuda Lei Wen Xuying Zhang Shengli Zhao
Keywords	Animals Mice Neurons Mice, Transgenic Optogenetics Rhodopsin Neural Pathways Nerve Net Action Potentials
Abstract	Here we characterize several new lines of transgenic mice useful for optogenetic analysis of brain circuit function. These mice express optogenetic probes, such as enhanced halorhodopsin or several different versions of channelrhodopsins, behind various neuron-specific promoters. These mice permit photoinhibition or photostimulation both in vitro and in vivo. Our results also reveal the important influence of fluorescent tags on optogenetic probe expression and function in transgenic mice.
Year of Publication	2013
Journal	Front Neural Circuits
Volume	7
Pages	160
Date Published	2013
ISSN	1662-5110
DOI	10.3389/fncir.2013.00160
PubMed ID	24324405
PubMed Central ID	PMC3840435
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