A DNA-based molecular probe for optically reporting cellular traction forces.
Nat Methods
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| Abstract | We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can in principle be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins conjugated to fluorophore-quencher pairs that unfold and fluoresce when subjected to specific forces. We applied TPs to reveal that cellular traction forces are heterogeneous within focal adhesions and localized at their distal edges. |
| Year of Publication | 2014
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| Journal | Nat Methods
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| Volume | 11
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| Issue | 12
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| Pages | 1229-32
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| Date Published | 2014 Dec
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| ISSN | 1548-7105
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| DOI | 10.1038/nmeth.3145
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| PubMed ID | 25306545
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| PubMed Central ID | PMC4247985
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| Links | |
| Grant list | R01 EB008396 / EB / NIBIB NIH HHS / United States
R37 GM057035 / GM / NIGMS NIH HHS / United States
R01 GM057035 / GM / NIGMS NIH HHS / United States
GM065865 / GM / NIGMS NIH HHS / United States
HL115553 / HL / NHLBI NIH HHS / United States
P41 EB001046 / EB / NIBIB NIH HHS / United States
R01 HL115553 / HL / NHLBI NIH HHS / United States
T32 GM008294 / GM / NIGMS NIH HHS / United States
R01 EB000262 / EB / NIBIB NIH HHS / United States
GM57035 / GM / NIGMS NIH HHS / United States
EB00262 / EB / NIBIB NIH HHS / United States
GM74048 / GM / NIGMS NIH HHS / United States
Howard Hughes Medical Institute / United States
EB001046 / EB / NIBIB NIH HHS / United States
UH3 EB017103 / EB / NIBIB NIH HHS / United States
R01 GM065865 / GM / NIGMS NIH HHS / United States
T32 EB005583 / EB / NIBIB NIH HHS / United States
EB08396 / EB / NIBIB NIH HHS / United States
R01 GM074048 / GM / NIGMS NIH HHS / United States
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