A sequential strand-displacement strategy enables efficient six-step DNA-templated synthesis.
J Am Chem Soc
| Authors | |
| Keywords | |
| Abstract | We developed a sequential strand-displacement strategy for multistep DNA-templated synthesis (DTS) and used it to mediate an efficient six-step DTS that proceeded in 35% overall yield (83% average yield per step). The efficiency of this approach and the fact that the final product remains linked to a DNA sequence that fully encodes its reaction history suggests its utility for the translation of DNA sequences into high-complexity synthetic libraries suitable for in vitro selection. |
| Year of Publication | 2011
|
| Journal | J Am Chem Soc
|
| Volume | 133
|
| Issue | 26
|
| Pages | 9972-5
|
| Date Published | 2011 Jul 06
|
| ISSN | 1520-5126
|
| DOI | 10.1021/ja201361t
|
| PubMed ID | 21657248
|
| PubMed Central ID | PMC3125949
|
| Links | |
| Grant list | R01 GM065865 / GM / NIGMS NIH HHS / United States
R01GM065865 / GM / NIGMS NIH HHS / United States
Howard Hughes Medical Institute / United States
|