Interaction-dependent PCR: identification of ligand-target pairs from libraries of ligands and libraries of targets in a single solution-phase experiment.
J Am Chem Soc
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| Abstract | Interaction-dependent PCR (IDPCR) is a solution-phase method to identify binding partners from combined libraries of small-molecule ligands and targets in a single experiment. Binding between DNA-linked targets and DNA-linked ligands induces formation of an extendable duplex. Extension links codes that identify the ligand and target into one selectively amplifiable DNA molecule. In a model selection, IDPCR resulted in the enrichment of DNA encoding all five known protein-ligand pairs out of 67 599 possible sequences. |
| Year of Publication | 2010
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| Journal | J Am Chem Soc
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| Volume | 132
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| Issue | 44
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| Pages | 15522-4
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| Date Published | 2010 Nov 10
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| ISSN | 1520-5126
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| DOI | 10.1021/ja107677q
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| PubMed ID | 20949943
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| PubMed Central ID | PMC2974369
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| Grant list | R01 GM065865 / GM / NIGMS NIH HHS / United States
GM065865 / GM / NIGMS NIH HHS / United States
Howard Hughes Medical Institute / United States
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