High-yield synthesis of N-acetyllactosamine by regioselective transglycosylation.
Biochem Biophys Res Commun
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| Abstract | The synthesis of N-acetyllactosamine (D-Galp beta 1-4D-GlcpNAc) with very low contamination of its isomer N-acetyllactosamine (D-Galp beta 1-6D-GlcpNAc) was obtained by use of regioselective transglycosylation activity of beta-galactosidase from Bacillus circulans using lactose as the donor of D-Galp and D-GlcpNAc as the acceptor. The reaction was conducted at 15 degrees C and at pH 5.0. The incubation time was considerably reduced and the yield improved 100% with respect to the best results so far described in the literature. |
| Year of Publication | 1996
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| Journal | Biochem Biophys Res Commun
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| Volume | 219
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| Issue | 1
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| Pages | 6-13
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| Date Published | 1996 Feb 06
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| ISSN | 0006-291X
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| DOI | 10.1006/bbrc.1996.0172
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| PubMed ID | 8619828
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