Chromatin profiling by directly sequencing small quantities of immunoprecipitated DNA.
Nat Methods
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| Abstract | Chromatin structure and transcription factor localization can be assayed genome-wide by sequencing genomic DNA fractionated by protein occupancy or other properties, but current technologies involve multiple steps that introduce bias and inefficiency. Here we apply a single-molecule approach to directly sequence chromatin immunoprecipitated DNA with minimal sample manipulation. This method is compatible with just 50 pg of DNA and should thus facilitate charting chromatin maps from limited cell populations. |
| Year of Publication | 2010
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| Journal | Nat Methods
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| Volume | 7
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| Issue | 1
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| Pages | 47-9
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| Date Published | 2010 Jan
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| ISSN | 1548-7105
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| URL | |
| DOI | 10.1038/nmeth.1404
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| PubMed ID | 19946276
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| PubMed Central ID | PMC2862482
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| Grant list | R01 HG004401 / HG / NHGRI NIH HHS / United States
U54 HG004570-02 / HG / NHGRI NIH HHS / United States
R01 HG004401-03 / HG / NHGRI NIH HHS / United States
U54 HG004570-01 / HG / NHGRI NIH HHS / United States
R44 HG005279-01 / HG / NHGRI NIH HHS / United States
U54 HG004570 / HG / NHGRI NIH HHS / United States
Howard Hughes Medical Institute / United States
R44 HG005279 / HG / NHGRI NIH HHS / United States
U54 HG004570-03 / HG / NHGRI NIH HHS / United States
DP1 OD003958 / OD / NIH HHS / United States
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