Efficient CRISPR-Cas9-mediated genome editing in Plasmodium falciparum.
Nat Methods
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| Abstract | Malaria is a major cause of global morbidity and mortality, and new strategies for treating and preventing this disease are needed. Here we show that the Streptococcus pyogenes Cas9 DNA endonuclease and single guide RNAs (sgRNAs) produced using T7 RNA polymerase (T7 RNAP) efficiently edit the Plasmodium falciparum genome. Targeting the genes encoding native knob-associated histidine-rich protein (kahrp) and erythrocyte binding antigen 175 (eba-175), we achieved high (≥ 50-100%) gene disruption frequencies within the usual time frame for generating transgenic parasites. |
| Year of Publication | 2014
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| Journal | Nat Methods
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| Volume | 11
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| Issue | 9
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| Pages | 915-8
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| Date Published | 2014 Sep
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| ISSN | 1548-7105
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| URL | |
| DOI | 10.1038/nmeth.3063
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| PubMed ID | 25108687
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| PubMed Central ID | PMC4199390
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| Grant list | 1DP2OD007124 / OD / NIH HHS / United States
DP2 OD007124 / OD / NIH HHS / United States
5-T32-ES007020 / ES / NIEHS NIH HHS / United States
T32 GM008334 / GM / NIGMS NIH HHS / United States
T32 ES007020 / ES / NIEHS NIH HHS / United States
P30 ES002109 / ES / NIEHS NIH HHS / United States
DP1 MH100706 / MH / NIMH NIH HHS / United States
R01 DK097768 / DK / NIDDK NIH HHS / United States
5-T32-GM08334 / GM / NIGMS NIH HHS / United States
P30-ES002109 / ES / NIEHS NIH HHS / United States
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