Building the Connectivity Map of epigenetics: chromatin profiling by quantitative targeted mass spectrometry.

Methods

Authors	Amanda Creech Jordan Taylor Verena Maier Xiaoyun Wu Caitlin Feeney Namrata Udeshi Sally Peach Jesse Boehm Jeannie Lee Steven Carr Jacob Jaffe
Keywords	Humans Mass Spectrometry Genomics Proteomics Chromatin Protein Processing, Post-Translational Histones Epigenomics
Abstract	Epigenetic control of genome function is an important regulatory mechanism in diverse processes such as lineage commitment and environmental sensing, and in disease etiologies ranging from neuropsychiatric disorders to cancer. Here we report a robust, high-throughput targeted, quantitative mass spectrometry (MS) method to rapidly profile modifications of the core histones of chromatin that compose the epigenetic landscape, enabling comparisons among cells with differing genetic backgrounds, genomic perturbations, and drug treatments.
Year of Publication	2015
Journal	Methods
Volume	72
Pages	57-64
Date Published	2015 Jan 15
ISSN	1095-9130
URL
DOI	10.1016/j.ymeth.2014.10.033
PubMed ID	25448295
PubMed Central ID	PMC4300274
Links
Grant list	R21 DA025720 / DA / NIDA NIH HHS / United States U01 CA164186 / CA / NCI NIH HHS / United States R21 DA025720-02 / DA / NIDA NIH HHS / United States U01 CA164186-02 / CA / NCI NIH HHS / United States