Efficient, continuous mutagenesis in human cells using a pseudo-random DNA editor.
Nat Biotechnol
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| Abstract | Here we describe TRACE (T7 polymerase-driven continuous editing), a method that enables continuous, targeted mutagenesis in human cells using a cytidine deaminase fused to T7 RNA polymerase. TRACE induces high rates of mutagenesis over multiple cell generations in genes under the control of a T7 promoter integrated in the genome. We used TRACE in a MEK1 inhibitor-resistance screen, and identified functionally correlated mutations. |
| Year of Publication | 2020
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| Journal | Nat Biotechnol
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| Volume | 38
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| Issue | 2
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| Pages | 165-168
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| Date Published | 2020 02
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| ISSN | 1546-1696
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| DOI | 10.1038/s41587-019-0331-8
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| PubMed ID | 31844291
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| Grant list | DP5-OD024583 / U.S. Department of Health & Human Services | National Institutes of Health (NIH) / International
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