New CRISPR-based research tool delivers results in an hour in a one-step reaction; Researchers share protocol and kits to advance research and move toward clinical validation.
The diagnostic platform CARMEN combines microfluidics with the CRISPR-based detection technology SHERLOCK, and could one day be used for public health efforts.
New technique enables SHERLOCK to detect a virus directly in bodily fluids, eliminating a step that required lab equipment and expanding the platform’s potential to quickly and cheaply track pathogens such as Ebola, Zika, and Lassa fever anywhere in the world
A strip of paper can now indicate presence of pathogens, tumor DNA, or any genetic signature of interest. 100-fold greater sensitivity, the ability to detect multiple targets at once, and other new features further enhance SHERLOCK's power for detecting genetic signatures.