Selection of unique Escherichia coli clones by random amplified polymorphic DNA (RAPD): Evaluation by whole genome sequencing.

J Microbiol Methods

Authors	Karen Nielsen Paul Godfrey Marc Stegger Paal Andersen Michael Feldgarden Niels Frimodt-Møller
Keywords	Reproducibility of Results High-Throughput Nucleotide Sequencing Genome, Bacterial Escherichia coli Feces Escherichia coli Infections Random Amplified Polymorphic DNA Technique Molecular Typing
Abstract	Identifying and characterizing clonal diversity are important when analysing fecal flora. We evaluated random amplified polymorphic DNA (RAPD) PCR, applied for selection of Escherichia coli isolates, by whole genome sequencing. RAPD was fast, and reproducible as screening method for selection of distinct E. coli clones in fecal swabs.
Year of Publication	2014
Journal	J Microbiol Methods
Volume	103
Pages	101-3
Date Published	2014 Aug
ISSN	1872-8359
URL
DOI	10.1016/j.mimet.2014.05.018
PubMed ID	24912108
PubMed Central ID	PMC4166437
Links
Grant list	HHSN272200900018C / AI / NIAID NIH HHS / United States U19 AI110818 / AI / NIAID NIH HHS / United States HHSN272200900018C / PHS HHS / United States